Dr Andrew Pugh1, Dr Carl Wardhaugh1, Celine Mercier1, Dr Justine Larrouy2, Dr Simon Bulman2, Dr Andrew Cridge1
1Scion, Rotorua, New Zealand, 2Plant & Food Research, Christchurch, New Zealand
Biography:
Andrew Cridge is a molecular entomologist with experience in biosecurity, biocontrol, insect genomics and genome evolution. His research focuses on developing integrated and sustainable solutions to detect and manage invasive insects in New Zealand’s native and productive ecosystems. He is currently researching centres on developing novel eDNA sampling and detection methods to improve terrestrial biosecurity monitoring. Andrew joined Scion in December 2020 as the Research Portfolio Leader for “Trees for High Volume Wood Products”. He is a graduate of Lincoln University (BSc (Hons)) and Otago University (PhD).
Abstract:
Environmental DNA (eDNA) is revolutionising biosecurity monitoring, offering an efficient method for detecting invasive pests and diseases. While eDNA monitoring in aquatic environments has been implemented, its application in terrestrial biosecurity remains underdeveloped. This study aimed to develop an air filtration eDNA aggregation methodology for early detection of invasive species to enable cost-effective and scalable tools for landscape-scale biosecurity monitoring at high-risk sites.
Our study tested the practicality of low-cost, portable, air filtering eDNA aggregators for rapid detection of insect species at biosecurity transition facilities at the Port of Tauranga. We evaluated their ability to capture and identify a diverse range of insect species from airborne particles, demonstrating their potential for real-world biosecurity applications.
The air filtration eDNA aggregation technique successfully captured and identified a wide range of insect taxa. The method demonstrated high sensitivity in detecting common and rare insect species in the sampled areas. Comparative analysis with light trap studies revealed that the eDNA approach provided comparable detection rates for most insect groups, specifically the more abundant taxa, including Lepidoptera (moths and butterflies) and Diptera (flies).
Our findings underscore the potential of terrestrial eDNA monitoring to enhance biosecurity surveillance. However, further research and collaboration are crucial to refine these techniques and integrate them into national biosecurity strategies.