Dr PORNTEP Punnarak1, Dr. Sureerat Tang2, Dr. Sirithorn Janpoom2, Dr. Sirikan Prasertlux2, Dr. Bavornlak Khamnamtong2, Dr. Wuthiwong Wimolsakcharoen3, Dr. Tongchai Thitiphuree1, Ms. Chiratthakan Getwech4, Ms. Phanachit Dhanasin4, Dr. Sirawut Klinbunga2, Professor Dr. Padermsak Jarayabhand5
1Aquatic Resources Research Institute, Chulalongkorn University, Bangkok, Thailand, 2Aquatic Molecular Genetics and Biotechnology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Thailand, 3264/118 The Tree Charan 30, Charansanitwong Rd., Ban Chang Loh, Bangkok Noi, Thailand, 4PTT Exploration and Production Public Company Limited, Bangkok, Thailand, 5Interdiciplinary Graduate Program in Maritime Administration (MARAD), Graduate School, Chulalongkorn University, Bangkok, Thailand
Biography:
Porntep Punnarak was born in Ratchaburi Province, Thailand in September 1979. He got his bachelor’s and master’s degree in marine science from Chulalongkorn University, Thailand in April 2002 and April 2005, respectively. He got his Ph.D in applied marine bioscience from Tokyo University of Marine Science and Technology, Tokyo, Japan in March 2012.
He is a Professional researcher at the Aquatic Resources Research Institute, Chulalongkorn University, Thailand, since 2013. His current research interests are in the fields of biology, taxonomy and ecology of plankton in coastal and marine environments, aquaculture of microalgae and zooplankton, and the application of molecular techniques including sanger sequencing and eDNA in marine biodiversity and ecosystem services.
Abstract:
Microbes play a significant role in different ecosystems, including marine environments. Here, microbial biodiversity in water and soil near the petroleum platforms located in Gulf of Thailand were preliminary examined using 16S rDNA-V3+V4. Water and Soil samples were collected from two central processing platforms (CPPs) and one wellhead platform (WHP). The water sample was also collected from a low-disturbed reference site. The 16S rDNA-V3+V4 detected 39 and 54 phyla from water and soil samples. The number of genus and species in water (199–382 and 217–393) were much lower than from soil (563–794 and 603–894) with overall identified species of 800 and 1580, respectively. Generally, slightly greater numbers of microbial species were detected in water samples of the platforms than the reference site. Disregarding unclassified taxa, Cyanobacteraceae was the most abundant family in water followed by Actinomarinales, Clade I, Sar86_clade. In soil, Thermoanaerobaculaceae, Kiloneillaceae, and Phycisphaeraceae predominated. Shannon diversity and Simpson evenness indices were significantly different in water and soil from different platforms (P < 0.05). Anosim and PERMANOVA analyses revealed significantly different of beta-diversity between platforms previously analyzed by PCoA and NDMS (P < 0.01). Dissolved oxygen seems to be an important factor for microbial biodiversity in water (P < 0.05). Key oil-degrading bacteria (e.g., Oceanospirillaceae, Piscirickettsiaceae, Desulfobacteriaceae, Colwellia and Cycloclasticus) were detected. The use of eDNA is promising for monitoring and assessing the abundance of bacterial communities and also serves as a tool to understand the ecological role of microbes in regulating marine environment.